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AP Biology Free Response Questions

The best way to get better at FRQs is practice. Browse through dozens of practice AP Biology FRQs to get ready for the big day.

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  • Unit 1: Chemistry of Life (31)
  • Unit 2: Cell Structure and Function (23)
  • Unit 3: Cellular Energetics (27)
  • Unit 4: Cell Communication and Cell Cycle (31)
  • Unit 5: Heredity (25)
  • Unit 6: Gene Expression and Regulation (22)
  • Unit 7: Natural Selection (60)
  • Unit 8: Ecology (31)
Unit 1: Chemistry of Life

Analyzing the Impact of Glucose Structural Conformation on Metabolic Reaction Rates

Researchers are investigating whether the structural conformation of glucose (cyclic vs. straight-ch

Medium

Atomic Structure and Isotopes Analysis

Investigate the nature of atomic structure by analyzing a table that summarizes various isotopes of

Medium

Carbohydrate Structure and Monosaccharide Energy Utilization

A nutritionist is investigating the energy yield differences between monosaccharides and disaccharid

Medium

Cell Membrane Dynamics and Phospholipid Density

Cell membranes are composed primarily of a phospholipid bilayer, whose organization is crucial for t

Medium

Comparative Analysis of Carbohydrate Storage

Two plant species utilize different carbohydrate storage molecules. Species A primarily stores energ

Hard

Dehydration Synthesis and Hydrolysis Reactions

Discuss how cells use dehydration synthesis and hydrolysis reactions to build and break down polymer

Easy

Effects of pH and Temperature on Protein Structure

This FRQ examines how protein structure is influenced by pH and temperature. You will describe the m

Hard

Enzyme Activity and Environmental pH

Analyze how changes in pH affect enzyme activity and structure.

Medium

FRQ 1: Elements in Biological Molecules

Discuss the role of key elements (O, C, H, N) in forming biological molecules and explain the import

Easy

FRQ 5: Interpreting a Diagram of Hydrogen Bonding in Water

The diagram provided depicts water molecules interacting via hydrogen bonds. Analyze the diagram and

Hard

FRQ 10: Levels of Protein Structure

Proteins exhibit four levels of structure that are essential for their function. Analyze these struc

Medium

FRQ 19: Comparative Analysis of Bonding in Biological Systems

Contrast hydrogen bonds and covalent bonds in terms of their strength, stability, and roles in biolo

Medium

Interactions Among Biomolecules: A Case Study

This question examines the variety of non-covalent interactions that underlie the assembly of comple

Extreme

Investigating Nucleic Acid Polymerization via Hydrolysis Reaction

A researcher studies the hydrolysis of RNA under acidic conditions to break it down into individual

Medium

Lipid Saturation and Membrane Fluidity

Evaluate the relationship between fatty acid saturation and the fluidity of cell membranes.

Hard

Lipids and Membrane Structure

Discuss the roles of phospholipids and cholesterol in maintaining membrane fluidity and integrity, a

Medium

Nitrogen in Macromolecules

Nitrogen is an essential element in many organic molecules. (a) Explain why nitrogen is important i

Easy

Nucleic Acid Formation and Hydrogen Bonding in DNA

DNA is synthesized through dehydration reactions that form phosphodiester bonds, and its double-heli

Hard

Nucleic Acid Polymerization and Base Pairing

Examine the molecular processes involved in DNA replication, focusing on polymerization and base pai

Extreme

Nucleic Acid Structure and Function

Compare the structural features of DNA and RNA and discuss how these differences are related to thei

Easy

Nucleic Acids: Structure, Function, and Polymerization

Explore the structure and synthesis of nucleic acids. Analyze how nucleotide properties contribute t

Hard

Organic Molecule Synthesis: Dehydration Synthesis

A biochemist investigates the formation of polysaccharides from monosaccharides through dehydration

Easy

Organic Molecules: Dehydration Synthesis and Hydrolysis

Examine the processes of dehydration synthesis and hydrolysis in the formation and breakdown of poly

Medium

Polymerization and Hydrolysis in Biological Systems

This question addresses the processes of dehydration synthesis and hydrolysis in the formation and b

Medium

Protein Structure and Folding

Proteins have a complex hierarchy of structure that is critical to their function. (a) Describe the

Easy

Protein Structure and Function

Analyze the hierarchical levels of protein structure and the interactions that stabilize them.

Medium

Protein Structure and Function: From Primary to Quaternary Structures

In this question, you will outline protein structure from the primary sequence to quaternary arrange

Hard

Understanding the Role of Molecular Components in Cell Signaling

A study investigates how cholesterol-derived hormones are produced and how molecular structure influ

Medium

Utilizing Isotope Gradients to Study Metabolism

This question focuses on the application of isotopic labeling for tracking metabolic pathways. You w

Extreme

Water in Polymer Hydrolysis Reactions

Analyze the role of water in the hydrolysis of polymers.

Medium

Water's Anomalous Properties and Ecological Impact

This question explores the unusual properties of water, particularly its expansion upon freezing, an

Extreme
Unit 2: Cell Structure and Function

Analyzing Protein Synthesis: The Role of Ribosomes

A molecular biologist is investigating protein synthesis by examining the role and structure of ribo

Easy

Aquaporin Overexpression and Water Transport

Design an experiment to determine how overexpression of aquaporin proteins affects water transport i

Medium

Cell Membrane Signaling and Receptor Function

Signal transduction is initiated at the cell membrane by receptor proteins that interact with extrac

Extreme

Cell Surface Area-to-Volume Ratio and Its Effects on Cellular Efficiency

In the study of cell physiology, the relationship between surface area and volume has important impl

Medium

Chloroplast Photosynthesis Efficiency Experiment

An experiment was established to study the effect of light intensity on photosynthetic efficiency in

Medium

Comparative Analysis of Prokaryotic and Eukaryotic Cells

The following table lists key features of prokaryotic and eukaryotic cells. Analyze the differences

Medium

Comparing Prokaryotic and Eukaryotic Cells

This FRQ examines the differences between prokaryotic and eukaryotic cells, using comparative data.

Easy

Diffusion through Aquaporins: Rate Calculations

Fick's first law of diffusion is given by $$J = -D * \frac{dC}{dx}$$, where J is the diffusion flux,

Extreme

Fundamentals of Diffusion and Osmosis

An experiment is conducted to observe the movement of solutes and water across cell membranes, highl

Easy

Golgi Complex: Processing and Packaging Proteins

The Golgi complex is essential for modifying, sorting, and packaging proteins for transport. Answer

Medium

Graphical Analysis of Osmosis in Plant Cells

This question involves analyzing a graph that displays water potential in plant cells under hyperton

Medium

Heat Exchange and Surface Area-to-Volume Ratio in Organisms

Design an experiment to investigate how heat-exchange rates vary in organisms with different surface

Hard

Interdependence of Cellular Structures in Transport and Metabolism

Cells rely on a network of organelles and structures to coordinate transport and metabolic processes

Extreme

Mechanisms of Endocytosis

This question explores the different types of endocytosis and their functional significance.

Easy

Mitochondria: Energy Conversion and ATP Production

The table below presents experimental data on ATP production rates by isolated mitochondria at vario

Medium

Mitochondrial Function and Cellular Respiration

A biologist obtains experimental data showing oxygen consumption in cells under varying concentratio

Medium

Nuclear Organization and Function

The nucleus is central to eukaryotic cell function. (a) Explain the role of the nucleus in gene reg

Medium

Osmoregulation and Water Potential in Plant Cells

This question integrates quantitative calculation using water potential with qualitative analysis to

Medium

Passive Transport: Simple and Facilitated Diffusion

Passive transport in cells occurs via simple diffusion or facilitated diffusion. Answer the followin

Hard

Prokaryotic vs. Eukaryotic Growth Rates

Design an experiment to compare the growth rates of a prokaryotic organism (e.g., a bacterium) and a

Easy

Sodium-Potassium Pump: Active Transport and Energy Consumption

A biochemist is investigating the sodium-potassium pump in animal cells. The pump moves 3 Na+ ions o

Medium

Surface Area-to-Volume Ratio in Cells

This question examines how the surface area-to-volume ratio affects cell function, especially with r

Medium

Surface Area-to-Volume Ratio in Organisms

An experiment was designed to examine how organism size affects heat exchange with the environment u

Easy
Unit 3: Cellular Energetics

Advanced Enzyme Energy Diagrams under Variable Conditions

This advanced problem examines how enzyme-catalyzed energy diagrams are altered under different temp

Extreme

Aerobic Respiration and ATP Yield

A researcher studies aerobic respiration to understand the contributions of different stages of gluc

Medium

ATP Yield in Cellular Respiration Processes

Refer to the table below listing ATP and electron carrier yields from different stages of cellular r

Medium

Cellular Respiration Stages and Energy Production

Describe the four stages of cellular respiration, detailing the key reactions and net production of

Hard

Chloroplast Structural Influence on Photosynthetic Efficiency

Chloroplast structure, particularly the organization of thylakoids into grana, affects photosyntheti

Hard

Chloroplast Structure and Function

Examine the provided diagram of a chloroplast and identify key structures involved in photosynthesis

Medium

Comparing Competitive vs Noncompetitive Inhibition

Answer the following questions regarding enzyme inhibition using the provided data.

Medium

Coupled Reactions and Reaction Mechanisms

In cells, unfavorable (endergonic) reactions are often driven by coupling them with favorable (exerg

Medium

Designing an Experiment on Allosteric Regulation

Design a controlled experiment to test the effect of an allosteric inhibitor on enzyme activity.

Hard

Effect of Inorganic Cofactor Concentrations on Enzyme Kinetics

Design an experiment to assess how varying concentrations of an inorganic cofactor (e.g., $$Mg^{2+}$

Medium

Effect of pH on Enzyme Conformation and Activity

A laboratory experiment measured the activity of an enzyme at various pH levels. The following table

Medium

Effect of Temperature on Enzyme Activity

A researcher investigates the effect of temperature on the activity of catalase by measuring the rat

Medium

Environmental Impact on Enzyme Function

This question assesses how extreme environmental conditions affect enzyme structure and function.

Medium

Enzyme Kinetics Analysis

A researcher conducts an experiment where the rate of an enzyme reaction is measured at varying subs

Easy

Enzyme Regulation and Inhibition Mechanisms

Cells tightly regulate enzyme activity to maintain metabolic balance. Evaluate the mechanisms of enz

Medium

Enzyme Saturation and Substrate Concentration

The table below shows how reaction rate varies with increasing substrate concentration. Use the data

Easy

Examination of Energy Coupling in Chloroplast ATP Synthesis

A research team designed an experiment to study the coupling of proton gradient formation to ATP syn

Hard

Feasibility of Reaction Coupling in Metabolic Pathways

In a coupled reaction scenario, one reaction releases $$-40*kJ/mol$$ while a second reaction require

Easy

Impact of Inhibitors on Metabolic Pathways

A researcher analyzes the impact of enzyme inhibitors on a metabolic pathway by examining changes in

Hard

Impact of Unintended Inhibition on Hexokinase Activity Measurements

A student designed an experiment to measure the kinetics of hexokinase by monitoring the rate of glu

Medium

Investigating the Effects of pH and Temperature on Enzyme Kinetics

A researcher aims to study the combined effects of pH and temperature on the kinetic parameters of a

Extreme

Investigating the Rate of Photosynthesis

Using experimental data, analyze how light intensity affects the rate of oxygen production in aquati

Hard

Reaction Coupling and ATP Synthesis

Reaction coupling is central to cellular metabolism. Using ATP hydrolysis as an example, explain how

Medium

Role of Cofactors in Enzyme Activity

Design an experiment to compare enzyme activity in the presence and absence of a cofactor (e.g., $$M

Easy

The Calvin Cycle and Carbon Fixation Pathways

This question examines the Calvin-Benson cycle along with alternative carbon fixation pathways (C3,

Hard

Thermodynamic Constraints on Reaction Coupling

This question explores the thermodynamic basis for coupling reactions in metabolism.

Medium

Thermodynamics in Cellular Processes

A cellular biologist is studying how cells maintain order despite continuous energy transformations.

Medium
Unit 4: Cell Communication and Cell Cycle

Assessing Ligand-Gated Ion Channel Response

A researcher investigates the response of neuronal cells to acetylcholine by measuring sodium ion in

Medium

Cascading Effects of Ligand-Receptor Binding Failures

A mutation in a receptor protein leads to impaired ligand binding efficiency, causing a significant

Hard

Cell Cycle Regulation and Cancer

This question addresses the regulatory mechanisms of the cell cycle and the potential consequences w

Hard

Chemical Inhibition of Ligand-Gated Ion Channels

Design an experiment to test how a chemical inhibitor affects the functioning of ligand-gated ion ch

Hard

Chemotaxis in Unicellular Organisms

A recent study claims that chemotaxis in unicellular organisms is primarily controlled by the intera

Easy

Chemotaxis in Unicellular Organisms Experiment

Design an experiment to investigate how a unicellular organism's movement (taxis) is influenced by n

Medium

Cyclin-CDK Complex and Its Regulation

Cyclin-CDK complexes are essential for the regulated progression of the cell cycle. A researcher is

Medium

Effects of Environmental Stress on Cell Signaling and Cycle Regulation

A study examines the impact of a high concentration of an environmental toxin on kidney cells, asses

Hard

Experimental Analysis of Signal Pathways

The table below shows experimental data relating ligand concentration to the relative production of

Hard

Experimental Design: Identifying Feedback Loop Mechanisms

A researcher is studying a metabolic pathway suspected to be regulated by a negative feedback loop.

Hard

Feedback Inhibition in Hormone Signaling

In an investigation of insulin's role in blood glucose regulation, a researcher treats isolated live

Easy

Feedback Loops and Homeostatic Regulation

Feedback systems in biological organisms help maintain homeostasis. Evaluate a negative feedback loo

Medium

Feedback Loops in Glucose Regulation

The regulation of blood glucose levels involves negative feedback mechanisms through hormones such a

Easy

Feedback Regulation in Cellular Signaling

This question explores the concepts of positive and negative feedback in cellular signal transductio

Medium

Integrative Analysis: Linking Cell Communication and Cell Cycle Regulation

The interplay between external signals and cell cycle regulators is critical for proper cell divisio

Extreme

Interphase Dynamics and DNA Replication

It is claimed that the S phase of interphase is critical for accurate DNA replication and that error

Medium

Investigation of Amplification in Signal Transduction Cascades

Signal amplification allows a small initial stimulus to generate a large cellular response. A resear

Hard

Investigation of Mitotic Spindle Assembly and Function

The proper function of the mitotic spindle is essential for faithful chromosome segregation during c

Hard

Ligand Specificity in Receptor Binding Assays

A researcher aims to investigate the specificity of a ligand for a ligand-gated ion channel in neuro

Hard

Ligand-Gated Ion Channels and Muscle Contraction

A study examines the role of acetylcholine in muscle contraction via ligand-gated ion channels. The

Easy

Ligand-Gated Ion Channels in Neuromuscular Function

This question focuses on the role of ligand-gated ion channels in initiating muscle contraction and

Medium

Mitosis Errors and Genetic Consequences

Errors during mitosis can lead to significant genetic abnormalities. A researcher is studying the fr

Hard

Negative Feedback in Cellular Signaling

A study on hormone regulation claims that negative feedback pathways stabilize hormone levels by red

Medium

Pathway Crosstalk Analysis in Fibroblasts

A researcher explores potential crosstalk between the MAP kinase pathway and the PI3K/Akt pathway in

Extreme

Plant Cell Signal Transduction

This question investigates how plant cells use signal transduction to respond to environmental stimu

Medium

Receptor Specificity and Drugs Targeting Signal Transduction Pathways

Pharmaceutical agents can target receptor signaling pathways to modulate cellular responses. Evaluat

Easy

Signal Amplification in Cell Communication

Signal transduction cascades allow cells to amplify a small external signal. Using the concept of si

Easy

Signal Transduction Cascades: Ligand-Receptor Binding

Design an experiment to measure the activation of a receptor by a specific ligand and the resulting

Hard

Signal Transduction Pathway Analysis in Eukaryotic Cells

A research lab studies a hormone signaling pathway in eukaryotic cells. In this pathway, the hormone

Medium

Temporal Dynamics of Mitosis Phases

This question investigates the timing of mitotic phases and the implications of phase duration on th

Easy

Tumor Suppressor Genes and Apoptotic Response

Researchers compare the response of wild type cells and cells with a mutated p53 gene (a key tumor s

Hard
Unit 5: Heredity

Analyzing Pedigree Data to Determine Inheritance Patterns

A genetic counselor is analyzing a pedigree chart showing the inheritance pattern of a particular tr

Medium

Application of Mendelian Principles in Plant Breeding

A plant breeder performs a cross between a heterozygous tall, yellow-flowered plant (TtYy) and a sho

Medium

Chromosome Number Analysis in Mitosis and Meiosis

A diploid cell has a chromosome number of $$2n = 6$$. Answer the following:

Easy

Designing a Mendelian Genetics Experiment

Design an experiment using pea plants to test the Law of Segregation. Answer the following: (a) Dev

Hard

Designing a Recombination Mapping Experiment

A geneticist seeks to map the order and distances between three genes on a chromosome. The experimen

Hard

Evolutionary Implications of Genetic Variation from Meiosis

Discuss how genetic variation generated during meiosis contributes to evolutionary adaptation in pop

Medium

Examining Dihybrid Cross and Independent Assortment in Pea Plants

Design an experiment using a dihybrid cross in pea plants to test Mendel’s Law of Independent Assort

Medium

Exploring X-Chromosome Inactivation and Barr Bodies

Researchers study female mammals to understand X-chromosome inactivation. They observe the presence

Easy

Gamete Production in Spermatogenesis and Oogenesis

A male's primary spermatocyte produces 4 functional sperm cells, whereas a female's primary oocyte p

Easy

Haploids Versus Diploids: Comparative Analysis

Cells exist in haploid and diploid states in living organisms. Using your understanding of chromosom

Easy

Investigation of the Law of Segregation in Pea Plants

A botanist studying the inheritance of pea plant height observes that self-pollinating the F1 genera

Medium

Maternal Inheritance of Mitochondrial Traits in Plants

Design an experiment to investigate the maternal inheritance of mitochondrial traits in a plant spec

Medium

Monohybrid Cross Analysis

Mendel's early work with pea plants laid the groundwork for understanding dominant and recessive tra

Medium

Monohybrid Cross Punnett Square Analysis

Analyze a monohybrid cross between two heterozygous individuals (Tt x Tt) for a trait where T is dom

Easy

Monohybrid Cross: Tall vs Short Pea Plants

This problem explores Mendel's law of dominance through a monohybrid cross between a heterozygous ta

Easy

Non-Mendelian Inheritance: Codominance in Flower Color

A researcher is investigating flower color in a plant species known to have codominant alleles for r

Easy

Pedigree Analysis in Genetic Inheritance

Pedigree charts are a useful tool for understanding inheritance patterns. Answer the following: (a)

Medium

Pedigree Chart Analysis

A pedigree chart is provided showing the inheritance of a trait across several generations. Analyze

Medium

Probability Calculations in Genetics

Probability rules are often used to predict outcomes in genetic crosses. Using the provided probabil

Medium

Recombinant Frequencies and Genetic Map Construction

Using recombination frequencies is a standard method for constructing genetic maps. In this scenario

Extreme

Recombination Frequency and Gene Mapping

Consider three linked genes A, B, and C. The recombination frequency between A and B is 15% and betw

Hard

Sex-Linked Inheritance in Pedigree Data

A geneticist analyzed a set of pedigree data for a trait suspected to be X-linked. The table below s

Medium

Test Cross Analysis in Mendelian Inheritance

A tall pea plant exhibits the dominant phenotype, but its genotype is unknown—it could be homozygous

Medium

Test Cross Analysis in Pea Plants

A tall pea plant has an ambiguous genotype. Describe how a test cross with a homozygous recessive (t

Medium

Understanding Sex-linked Traits and X Inactivation

Discuss the inheritance patterns of X-linked recessive traits (such as color blindness) and the role

Medium
Unit 6: Gene Expression and Regulation

Analysis of Mutational Impacts on Protein Structure

A molecular biologist is studying the effects of different point mutations on protein structure. The

Easy

Antiparallel DNA and Replication Dynamics

Discuss how the antiparallel nature of DNA influences the dynamics of replication.

Easy

Cell-Free Translation System: Ribosome Binding Site Error

A researcher develops a cell-free system to study translation by combining purified mRNA, ribosomes,

Medium

Central Dogma and Its Exceptions

Discuss the central dogma of molecular biology along with notable exceptions observed in certain org

Easy

Comparative Analysis of Gene Expression

Discuss the differences and implications of gene expression processes in prokaryotic and eukaryotic

Hard

FRQ 11: Transcription Factor Binding and Promoter Efficiency

Researchers have engineered a synthetic promoter to enhance gene expression in cultured cells. A rep

Hard

Genome Packaging and Chromatin State Effects on Transcription

Researchers are studying how chromatin structure impacts gene expression. In this experiment, cells

Medium

Genomic Organization and Chromatin Structure

Discuss how genomic organization affects gene expression.

Easy

Interplay of Transcription, RNA Processing, and Translation

Examine the sequential processes from transcription to translation and discuss how errors in RNA pro

Medium

Mutation Frequencies and Genetic Variation

Researchers collected data on mutation frequencies in a population after exposure to environmental s

Hard

Recombinant DNA and Ethical Implications

Explore the process of creating recombinant DNA and discuss its scientific benefits alongside potent

Hard

Recombinant DNA and Gene Cloning

A biotechnologist is engineering a plasmid to express a human gene (e.g., insulin) in bacteria.

Hard

Recombinant DNA and Ligation Efficiency

This question evaluates recombinant DNA techniques emphasizing ligation efficiency in cloning experi

Medium

Recombinant DNA Techniques and Applications

Discuss the methodology of creating recombinant DNA and analyze its real-world applications.

Hard

Recombinant DNA Technology

Discuss the process of creating recombinant DNA and its application in genetic engineering.

Medium

RNA Interference (RNAi) and Post-Transcriptional Regulation

This question explores the mechanism of RNA interference (RNAi) and its application in regulating ge

Medium

RNA Splicing and Transcript Variability

This question examines the impact of alternative RNA splicing on mRNA structure and protein diversit

Hard

Semiconservative DNA Replication and Labeling

Examine the semiconservative nature of DNA replication and the experimental evidence supporting it.

Hard

Transcription and RNA Processing Mechanisms

Researchers are comparing mRNA transcript lengths before and after splicing in eukaryotic cells. Usi

Hard

Translation Initiation and Elongation Experiment

A study examines how variations in ribosome number affect the rate of protein synthesis when mRNA co

Hard

Viral Life Cycles: Lytic vs Lysogenic

Contrast the lytic and lysogenic cycles of viral replication and analyze the unique role of reverse

Hard

Viral Replication and Horizontal Gene Transfer

Investigate the replication cycles of viruses and their role in horizontal gene transfer among bacte

Medium
Unit 7: Natural Selection

Analyzing Phylogenetic Relationships Through Molecular Data

A comparative study of DNA sequence divergence among species is represented by the graph provided. T

Hard

Artificial Selection in Crop Plants: Predicting Trait Frequency Changes

Investigate how artificial selection can alter the trait distribution in crop plants over one genera

Hard

Artificial Selection in Domestic Animals

A livestock breeder aims to increase milk production in cows through selective breeding. Design an e

Medium

Biogeographical Patterns and Evolution

Biogeography provides key evidence for evolution by examining the distribution of species across geo

Medium

Biogeography and Evolutionary Patterns

Biogeography provides important insights into the processes of speciation and evolutionary history.

Medium

Biogeography and Phylogenetic Trees

Biogeographical data and phylogenetic trees provide insights into evolutionary history. Answer the f

Hard

Climate Change and Evolutionary Selection

Climate change can act as a powerful selective force. Use the data provided to examine how changing

Hard

Constructing and Interpreting a Cladogram

A cladogram is a diagram that represents evolutionary relationships among species. Answer the follow

Medium

Darwin’s Natural Selection Observations

Analyze Darwin’s observations, including the production of excess offspring, competition, and differ

Easy

Drosophila Wing Shape Variation: Effects of Temperature on Morphology

A team of researchers is studying a population of Drosophila to investigate how temperature influenc

Medium

Effects of Gene Flow on Subpopulation Divergence

Two subpopulations of the same species are separated by a mountain range, but occasional migration (

Medium

Embryological Analysis of Vertebrate Development

In this experiment, a researcher examines the embryonic development of a range of vertebrate species

Medium

Environmental Change and Natural Selection in Lizards

Evaluate how sudden environmental changes can alter selective pressures within a lizard population.

Hard

Evaluating Lamarckian Inheritance Versus Darwinian Natural Selection

Jean-Baptiste de Lamarck proposed that acquired traits can be inherited, while Darwin’s theory empha

Medium

Evolution of Enzyme Function in Variable Environments

A study examines the catalytic activity of an enzyme extracted from a plant species and how it chang

Extreme

Evolutionary Consequences of Non-Random Mating in a Fish Population

In a coastal fish population, individuals preferentially choose mates with brighter coloration. Answ

Medium

Experimental Analysis of Mutation Rates in Bacteria

In laboratory experiments, mutation rates can be measured by exposing bacterial populations to varyi

Extreme

Fossil Record Analysis and Dating Techniques

Dating methods and fossil records are crucial for understanding evolutionary timelines. Delve into t

Extreme

Fossil Record and Evolutionary Trends

The table below shows fossil data from three different time periods, including age and dominant morp

Medium

Fossil Records and Morphological Homologies

This FRQ examines how fossil records and morphological homologies are used as evidence for common an

Medium

Founder Effect and Genetic Drift

Evaluate the consequences of the founder effect on genetic variability by analyzing allele frequency

Medium

Gene Flow and Local Adaptation in Fragmented Populations

Discuss the role of gene flow in shaping local adaptation in geographically fragmented populations.

Medium

Gene Flow and Speciation in Island Bird Populations

Bird populations on two nearby islands are occasionally connected by a land bridge, allowing for lim

Medium

Genetic Drift and the Bottleneck Effect

Using the data provided in the table, explain how a bottleneck event can impact genetic diversity an

Hard

Genetic Variability and Evolutionary Potential

Explain the importance of genetic variability in evolution and estimate the impact of mutation rate

Easy

Genetic Variability and Natural Selection Experiment

This FRQ explores the role of genetic variability in natural selection. Consider how variations with

Easy

Genetic Variability and the Hardy-Weinberg Equilibrium

Genetic variability is a foundation for evolution and is described mathematically by the Hardy-Weinb

Easy

Hardy-Weinberg Equilibrium and Evolutionary Forces

In a laboratory study of a beetle population, the following genotype frequencies were observed: $$p^

Hard

Impact of Environmental Changes on Evolutionary Fitness

This question explores how sudden environmental changes, such as temperature increases, impact evolu

Hard

Impact of Mutation, Genetic Drift, and Gene Flow

Mutations, genetic drift, and gene flow are non-selective mechanisms that can alter allele frequenci

Medium

Impacts of Mutation and Genetic Drift on Hardy-Weinberg Equilibrium

A population’s genotype frequencies were recorded before and after an event. Analyze the following d

Hard

Interpreting Phylogenetic Data Through Cladograms

Cladograms and phylogenetic trees help illustrate evolutionary relationships and common ancestry amo

Hard

Interpreting Phylogenetic Trees and Common Ancestry

Phylogenetic trees illustrate the evolutionary relationships among species. Analyze the provided phy

Medium

Lamarck vs Darwin: Comparative Analysis

Research on evolutionary theories continues to provoke discussion. Compare the historical theories o

Hard

Lamarck vs. Darwin: Mechanisms of Evolution

Contrast Darwin's theory of natural selection with Lamarck’s hypothesis of the inheritance of acquir

Easy

Measuring Evolutionary Fitness in Lizards

A researcher studying a desert lizard population records variations in tail length and associated re

Easy

Miller-Urey Experiment and Organic Compounds

Evaluate the significance of the Miller-Urey experiment in understanding the origin of life on Earth

Medium

Modeling Directional vs. Stabilizing Selection

This question requires analysis of selection patterns using a graph and a mathematical model. Determ

Extreme

Modeling Genetic Variation Using the Hardy-Weinberg Principle

A researcher is studying a fish population where a mutation conferring a sharp fin appears. The init

Medium

Modes of Natural Selection: Directional, Stabilizing, and Disruptive

Examine the different modes of natural selection based on the frequency distribution data provided i

Medium

Mutation Rates and Environmental Factors

Mutations introduce new genetic variation. Consider a gene with a length of 2000 base pairs and a mu

Extreme

Non-random Mating and Hardy-Weinberg Deviations

A table provides data on a population exhibiting non-random mating with an inbreeding coefficient $$

Extreme

Origins of Life and the RNA-World Hypothesis

Examine the evidence supporting the RNA-world hypothesis and its significance in understanding the o

Medium

Origins of Life: Analyzing the Oparin-Urey Experiment

The Oparin-Urey experiment aimed to simulate early Earth conditions in order to test hypotheses rega

Extreme

Origins of Life: Evaluating the RNA-World Hypothesis and the Miller-Urey Experiment

Examine the origins of life on Earth by discussing the RNA-world hypothesis and the significance of

Hard

Paleontological Evidence and Fossil Record Analysis

Analyze the fossil record data provided in the table to answer the following questions regarding the

Medium

Peppered Moths and Natural Selection: A Mathematical Analysis

This question examines the classic case of the peppered moths. Prior to industrialization, 50% of th

Hard

Peppered Moths: A Case Study in Directional Selection

Examine the changes in population frequencies of the peppered moth before and after industrializatio

Easy

Phylogenetic Trees and Common Ancestry

Researchers have compiled both molecular and fossil data to construct a phylogenetic tree that maps

Medium

Phylogenetic Trees and Common Ancestry

Examine the scenario described below regarding relationships among four species and their common anc

Medium

Phylogenetics and Cladistics: Inferring Evolutionary Relationships

Consider a simplified phylogenetic scenario in which species A and B form a clade based on shared de

Medium

Population Bottlenecks and Founder Effects

Population bottlenecks and founder effects are important examples of genetic drift. Evaluate how the

Medium

Population Genetics and Hardy-Weinberg Equilibrium

A biologist studied a population's genotype frequencies over 10 generations. The table below shows t

Medium

Reproductive Isolation and Speciation Mechanisms

Examine the processes that lead to speciation through reproductive isolation, focusing on both pre-z

Medium

Selection Types in Evolution

Compare and contrast directional, stabilizing, and disruptive selection; provide real-world or exper

Medium

Simulating Early Earth: The Miller-Urey Experiment

The Miller-Urey experiment attempted to simulate early Earth conditions and resulted in the formatio

Medium

Temperature Effects on Enzyme Activity and Evolutionary Adaptation

In an experiment, enzyme activity was measured at various temperatures to assess adaptive responses

Extreme

The Peppered Moth Case Study: Directional Selection

Examine the classic case of the peppered moth and answer the following questions:

Medium

The RNA World Hypothesis

Discuss the RNA world hypothesis and its significance in explaining the origins of life on Earth.

Medium

The RNA World Hypothesis and the Origins of Life

Experiments simulating early Earth conditions, such as the Miller-Urey experiment, provide support f

Extreme
Unit 8: Ecology

Agonistic Behavior and Competition in Animal Populations

Agonistic behaviors, which include aggressive interactions, are common in populations competing for

Hard

Altruism in Animal Groups

Data from behavioral studies on a social species indicate variations in the frequency of altruistic

Medium

Altruistic Behavior and Kin Selection

A behavioral study recorded the frequency of altruistic helping behaviors in relation to the coeffic

Medium

Animal Communication: Pheromones and Agonistic Behaviors

This FRQ focuses on the various ways animals communicate, emphasizing chemical signaling and social

Easy

Animal Communication: Pheromones and Social Hierarchies

Animal communication encompasses a variety of signals, including chemical cues like pheromones and c

Medium

Assessing Agonistic Behavior and Dominance Hierarchies

An observational study on a flock of birds provides data on aggressive interactions and shows that t

Hard

Circadian Rhythm Disruption in Nocturnal Mammals

A scientist investigates circadian rhythm patterns in a population of nocturnal bats. The experiment

Hard

Climate Change Effects on Population Dynamics and Distribution

Assess the potential impacts of climate change on species' population growth and geographic distribu

Extreme

Comparative Analysis of Circadian Rhythms in Plants and Animals

A researcher compared circadian rhythms in roosters and plants by recording their respective activit

Medium

Comparing Instinct and Learned Altruistic Behavior in Birds

Behavioral studies in birds show that certain behaviors such as warning calls can be either instinct

Easy

Ecological Succession in a Post-Volcanic Landscape

Investigate the process of primary ecological succession following a volcanic eruption, focusing on

Medium

Energy Transfer Analysis in Food Chains

Data from an ecosystem study provide the energy content at different trophic levels. Analyze the dat

Medium

Environmental Toxins and Biomagnification

A study assessed the concentration of a toxin at different trophic levels in an aquatic ecosystem. T

Hard

Examining Symbiotic Relationships: Mutualism, Commensalism, and Parasitism

A hypothesis proposes that ecosystem stability depends on the balance between mutualistic, commensal

Hard

Impact of Deforestation on Biodiversity and Biomass

A region experiencing deforestation was studied and the following data were recorded: Species richne

Hard

Impact of Deforestation on Ecosystem Productivity

An ecologist examines the effect of deforestation on ecosystem productivity in a tropical region by

Hard

Imprinting and Instinct: A Case Study

A researcher observed newly hatched chicks exposed to different moving objects during a critical per

Medium

Imprinting Timing and Responses in Ducklings

Researchers conducted an experiment on ducklings to study the imprinting process. In this experiment

Medium

Investigating Primary Succession After Disturbance

After a volcanic eruption, a newly formed land surface undergoes primary succession. Researchers cla

Medium

Keystone Species and Ecosystem Dynamics

Keystone species have a disproportionate effect on their ecosystems. Answer the following:

Hard

Learning Behaviors: Habituation and Imprinting in Birds

A behavioral ecologist wants to compare the processes of habituation and imprinting in birds by desi

Hard

Learning, Imprinting, and Habituation in Avian Species

Bird behavior is shaped by both innate mechanisms and experiences. In many bird species, imprinting

Medium

Photoperiodism and Plant Flowering

Plants rely on photoperiodism—the response to the duration of day and night—to time essential proces

Hard

Plant Tropisms: Phototropism vs Thigmotropism

A botanist is interested in determining whether the signaling pathways involved in phototropism (res

Medium

Population Ecology: Density-Dependent Factors and Limiting Resources

Using the table below, analyze how population density affects reproductive rate, $$r = \frac{(\text{

Hard

Population Growth Dynamics: Exponential vs Logistic Growth

A biologist studying a closed bacterial culture observes that the population initially grows exponen

Hard

Primary Succession Study on Rocky Substrates

A biologist investigates primary succession on a rocky substrate exposed by glacial retreat. The exp

Medium

Productivity in Ecosystems: Gross vs Net Primary Productivity

A researcher is comparing gross primary productivity (GPP) and net primary productivity (NPP) in a t

Hard

Simpson’s Diversity Index and Ecosystem Health

This FRQ assesses your understanding of biodiversity metrics, particularly Simpson’s Diversity Index

Hard

Thermoregulation in Endotherms vs. Ectotherms

Investigate how endotherms and ectotherms regulate their body temperatures and the impact of environ

Easy

Thigmotropism in Climbing Vines

An experiment was conducted to observe thigmotropism in a climbing vine. The vine was exposed to var

Medium

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FAQWe thought you might have some questions...
Where can I find practice free response questions for the AP Biology exam?
The free response section of each AP exam varies slightly, so you’ll definitely want to practice that before stepping into that exam room. Here are some free places to find practice FRQs :
  • Of course, make sure to run through College Board's past FRQ questions!
  • Once you’re done with those go through all the questions in the AP BiologyFree Response Room. You can answer the question and have it grade you against the rubric so you know exactly where to improve.
  • Reddit it also a great place to find AP free response questions that other students may have access to.
How do I practice for AP AP Biology Exam FRQs?
Once you’re done reviewing your study guides, find and bookmark all the free response questions you can find. The question above has some good places to look! while you’re going through them, simulate exam conditions by setting a timer that matches the time allowed on the actual exam. Time management is going to help you answer the FRQs on the real exam concisely when you’re in that time crunch.
What are some tips for AP Biology free response questions?
Before you start writing out your response, take a few minutes to outline the key points you want to make sure to touch on. This may seem like a waste of time, but it’s very helpful in making sure your response effectively addresses all the parts of the question. Once you do your practice free response questions, compare them to scoring guidelines and sample responses to identify areas for improvement. When you do the free response practice on the AP Biology Free Response Room, there’s an option to let it grade your response against the rubric and tell you exactly what you need to study more.
How do I answer AP Biology free-response questions?
Answering AP Biology free response questions the right way is all about practice! As you go through the AP AP Biology Free Response Room, treat it like a real exam and approach it this way so you stay calm during the actual exam. When you first see the question, take some time to process exactly what it’s asking. Make sure to also read through all the sub-parts in the question and re-read the main prompt, making sure to circle and underline any key information. This will help you allocate your time properly and also make sure you are hitting all the parts of the question. Before you answer each question, note down the key points you want to hit and evidence you want to use (where applicable). Once you have the skeleton of your response, writing it out will be quick, plus you won’t make any silly mistake in a rush and forget something important.